Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Forkhead Box 03A (also known as FOXO3A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human FOXO3A antibody. FOXO3A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human FOXO3A Antibody is added and binds to FOXO3A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FOXO3A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human FOXO3A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm